Dead-cell Removal

Products for removal of dying and dead cells from primary suspensions and cultures

Dead-Cert® Nanoparticles

Dead-Cert® Nanoparticles

Dead-Cert® Nanoparticles Super-paramagnetic nanoparticles for direct selective removal of dead and dying cells

Dead-Cert® Nanoparticles are specially prepared to allow direct removal of dying and dead cells from cell suspensions and cultures with minimum fuss and maximum efficiency. The active components, stably coupled to the surface of these 250 nm particles can selectively bind structures that are exposed on cells during and following death - either apoptosis or necrosis. Dead-Cert® Nanoparticles can often be used directly in the natural milieu of the cells – no need to change from culture medium to special buffers – and, to support the viability of your cells further, all procedures can be carried out at 0°C to 37°C. No columns are required for magnetic separation, just a simple magnet such as DC-002. Use YES medium (Yield-Enhancing Separation medium, DC-004) to optimize the separation procedure if your culture medium proves sub-optimal.

Click here for further information on principles of action of Dead-Cert® Nanoparticles

Click here for answers to frequently asked questions

Click here for customer reviews

Choose from 6 application-specific products. Each contains sufficient for 30 depletions.

Dead-Cert® CELL CULTURE

Nanoparticles for general use on cell populations that grow in suspension or as adherent monolayers. Boost cell growth rates and functional activities. Use for established cell lines or for establishing new lines from primary cells. Clean up debris from cell suspensions obtained from disrupted tissue samples.

Dead-Cert® CRYOPRESERVATION

Nanoparticles to improve quality of cell banks (when used to clean up cell populations prior to freezing) and to improve revival success and accelerate re-establishment of cell growth and productivity after thaw. Use for primary cells and cell lines (adherent and suspension). Improve reproducibility of stored samples and make cell banks go further.

Dead-Cert® ANTIBODY PRODUCTION

Nanoparticles for all your monoclonal antibody production needs. Improve hybridoma growth and productivity of individual cells by up to 3-fold. Shorter revival to bioreactor seed times. Scale up for substantial improvements in batch process. Produce more antibody faster.

Dead-Cert® STEM CELL

Nanoparticles for all your stem cell culture requirements. Suitable for murine and human stem cells and their differentiated progeny. Improve storage quality and growth. Clean up dead cells and debris from disrupted embryoid bodies. Accelerate establishment of stem cell lines.

Dead-Cert® RNA PREPARATION

Nanoparticles to improve the quality of RNA from animal cell samples – all cell lineages and all species from worms to mammmals. Dead and dying cells containing degraded RNA simply and effectively removed. Allows high quality RNA (suitable for gene expression profiling) to be produced even from poor cell samples, such as patient samples and tissue digests. Also improves DNA and protein quality.

Dead-Cert® DRUG SCREENING

Nanoparticles to improve the sensitivity of all cell-based assays through removal of non-viable cells which otherwise inhibit cell functions and increase background noise. Use for a wide variety of cell types and assay platforms, including immunocytochemistry, flow cytometry, cell migration, cell growth and toxicity tests. Vastly improves quality of assays performed on primary cells (such as patient samples) and sensitive cell lines.

Dead-Cert® Kits

Dead-Cert® Kits

Contains Dead-Cert® Nanoparticles (DC-001), Dead-Cert® microtube magnet (DC-002) and Dead-Cert® YES medium (Yield-Enhancing Separation medium) (DC-004). Sufficient for 30 depletions.

Dead-Cert® CELL CULTURE kit

Dead-Cert® CRYOPRESERVATION kit

Dead-Cert® ANTIBODY PRODUCTION kit

Dead-Cert® STEM CELL kit

Dead-Cert® RNA PREPARATION kit

Dead-Cert® DRUG SCREENING kit

Dead-Cert® Trial Kits

Dead-Cert® Trial Kits

Contains Dead-Cert® Nanoparticles (DC-001), Dead-Cert® microtube magnet (DC-002) and Dead-Cert® YES medium (Yield-Enhancing Separation medium) (DC-004). Sufficient for 5 depletions.

Dead-Cert® CELL CULTURE trial kit

Dead-Cert® CRYOPRESERVATION trial kit

Dead-Cert® ANTIBODY PRODUCTION trial kit

Dead-Cert® STEM CELL trial kit

Dead-Cert® RNA PREPARATION trial kit

Dead-Cert® DRUG SCREENING trial kit

Dead-Cert® YES Medium

Dead-Cert® YES Medium

Dead-Cert® YES medium (Yield-Enhancing Separation medium) is designed for use with Dead-Cert® Nanoparticles to optimise removal of non-viable cells and to maximise yields of viable cells. Sufficient for 30 depletions.

Dead-Cert® Microtube Magnet

Dead-Cert® Microtube Magnet

Neodymium magnet for efficient separation of Dead-Cert® Nanoparticles

The Dead-Cert® microtube magnet is ideal for performing rapid and efficient cell separations in 1.5 ml tubes.